Anti-Prostate Specific Antigen 抗体 [5A6] (HRP) (ab24466)

製品の概要

  • 製品名
    Anti-Prostate Specific Antigen antibody [5A6] (HRP)
    Prostate Specific Antigen 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP)
  • 標識
    HRP
  • 特異性
    Equimolar total PSA, epitope 5. No cross reactivity for human kallikrein 2.
  • アプリケーション
    適用あり: Sandwich ELISA, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human PSA antigen purified from semenal plasma).

  • ポジティブ・コントロール
    • This antibody gave a postive signal in Human Prostate tissue lysate and LNCaP whole cell lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187).

For the detection of free PSA, ab24466 can be used as a detection antibody in conjugation with ab10187. For total PSA detection ab24466 can be used as a capture antibody and ab10184 as a detection antibody.

WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • 配列類似性
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Anti-Prostate Specific Antigen antibody [5A6] (HRP) 画像

  • All lanes : Anti-Prostate Specific Antigen antibody [5A6] (HRP) (ab24466) at 1/5000 dilution

    Lane 1 : Human prostate tumor tissue lysate (adenocarcinoma) - total protein (ab30305)
    Lane 2 : LNCaP nuclear extract lysate (ab14857)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

Anti-Prostate Specific Antigen antibody [5A6] (HRP) (ab24466) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

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Thank you for contacting Abcam.

It appears our datasheet for ab24466 incorrectly states that 0.1% sodium azide is included in the storage buffer. Sodium azide is not present in the storage buffer since, as you mentioned, sodium azide affects H...

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That is correct: ab10184 for capture and ab24466 for detection. These are two different clones.  The detection antibody, ab24466, is conjugated to HRP. Some of the catalogue numbers of our other PSA antibodies are very similar so I will not even mentio...

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Thank you for your inquiry. Unfortunately, the isoelectric points of these antibodies have not been determined. According to my research, the pI of most monoclonal antibodies is around 6.1. The isoelectric point for horseradish peroxidase isozyme...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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