製品の概要

  • 製品名Anti-Prostaglandin I2 Receptor antibody
    Prostaglandin I2 Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Prostaglandin I2 Receptor
  • アプリケーション適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment with tag: RKAVFQRLKL WVCCLCLGPA HGDSQTPLSQ LASGRRDPRA PSAPVGKEGS CVPLSAWGEG QVEPLPPTQQ SSGSAVGTSS KAEASVACSL C, corresponding to amino acids 296-387 of Human Prostaglandin I2 Receptor

  • ポジティブ・コントロール
    • A549 whole cell lysate and human tonsil tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab60706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
IHC-P
WB
ICC/IF
  • 追加情報ELISA: Use at an assay dependent dilution.
    Note: The detection limit for ab60706 is approximately 0.1ng/ml as a capture antibody.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 41 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.
    • 配列類似性Belongs to the G-protein coupled receptor 1 family.
    • 翻訳後修飾Palmitoylation of either Cys-308 or Cys-311 is sufficient to maintain functional coupling to G(s) and signaling.
      Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • IP antibody
      • PGI receptor antibody
      • PGI2 receptor antibody
      • PI2R_HUMAN antibody
      • PRIPR antibody
      • Prostacyclin receptor antibody
      • Prostaglandin I2 (prostacyclin) receptor (IP) antibody
      • Prostaglandin I2 receptor antibody
      • Prostanoid IP receptor antibody
      • Ptgir antibody
      see all

    Anti-Prostaglandin I2 Receptor antibody 画像

    • ab60706 at 3µg/ml staining Prostaglandin I2 Receptor in human tonsil by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.
    • Anti-Prostaglandin I2 Receptor antibody (ab60706) + A549 whole cell lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG HRP at 1/2500 dilution
      Developed using the ECL technique

      Predicted band size : 41 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Anti-Prostaglandin I2 Receptor antibody (ab60706) 使用論文

    This product has been referenced in:
    • Larsson-Callerfelt AK  et al. Defective alterations in the collagen network to prostacyclin in COPD lung fibroblasts. Respir Res 14:21 (2013). ICC/IF ; Human . Read more (PubMed: 23406566) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us. Unfortunately the peptides used as the immunogens for these products are not available in our catalog.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advic...

    Read More

    I am sorry to hear this antibody is not providing satisfactory results.

    The details which you provided to us will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the ti...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"