製品の概要

  • 製品名
    Anti-Prostaglandin E Synthase antibody
    Prostaglandin E Synthase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Prostaglandin E Synthase
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to 14 amino acids near the N-terminus of Human Prostaglandin E Synthase.

  • ポジティブ・コントロール
    • Human testis tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).
  • 配列類似性
    Belongs to the MAPEG family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glutathione S transferase 1 like 1 antibody
    • MGC10317 antibody
    • MGST IV antibody
    • MGST1 L1 antibody
    • MGST1 like 1 antibody
    • MGST1-L1 antibody
    • MGST1L1 antibody
    • MGSTIV antibody
    • Microsomal glutathione S transferase 1 like 1 antibody
    • Microsomal glutathione S-transferase 1-like 1 antibody
    • Microsomal prostaglandin E synthase 1 antibody
    • Microsomal prostaglandin E synthase 1 antibody
    • mPGES 1 antibody
    • MPGES antibody
    • mPGES-1 antibody
    • p53 induced apoptosis protein 12 antibody
    • p53 induced gene 12 antibody
    • p53 induced gene 12 protein antibody
    • p53-induced gene 12 protein antibody
    • PGES antibody
    • PIG 12 antibody
    • PIG12 antibody
    • PP 102 antibody
    • PP 1294 antibody
    • PP102 antibody
    • PP1294 antibody
    • Prostaglandin E synthase antibody
    • PTGES antibody
    • PTGES_HUMAN antibody
    • TP53I12 antibody
    • Tumor protein p53 inducible protein 12 antibody
    see all

Anti-Prostaglandin E Synthase antibody 画像

  • ab62050 staining Prostaglandin E Synthase in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton-X and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 20 hours at 4°C. ab150075, a donkey anti-rabbit Alexa Fluor® 647- (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, staining Prostaglandin E Synthase with ab62050 at 10 µg/ml.

Anti-Prostaglandin E Synthase antibody (ab62050) 使用論文

This product has been referenced in:
  • Uchida K  et al. Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-a stimulation of synovial cells: an in vitro study. BMC Musculoskelet Disord 18:283 (2017). Read more (PubMed: 28668088) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Permeabilization
Yes - Triton-X
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3 cells)
Loading amount
20 µg
Specification
NIH3T3 cells
Treatment
24 hours IL1b stimulation (1ng/ml)
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells)
Loading amount
20 µg
Specification
A549 cells
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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