The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Extensive washing of the transfer membrane at each step to reduce non-specific background binding effects is required. Starting exposure time of 45 seconds is suggested. Lower dilutions of antibody should be used for alkaline phosphatase detection.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).