Anti-Prostaglandin E Receptor EP4 抗体 (SureLight® Allophycocyanin) (ab92763)

製品の概要

  • 製品名
    Anti-Prostaglandin E Receptor EP4 antibody (SureLight® Allophycocyanin)
    Prostaglandin E Receptor EP4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Prostaglandin E Receptor EP4 (SureLight® Allophycocyanin)
  • 標識
    SureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • 特異性
    ab92763 is reactive with EP4 receptor and non-reactive with EP1, EP2, and EP3 receptors.
  • アプリケーション
    適用あり: ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Human
    交差が予測される動物種: Rabbit, Cow, Chimpanzee, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    GSGRAGPAPKGSSLQVTFPSETLNLSEKCI

    , corresponding to C terminal amino acids 459-488 of Human Prostaglandin E Receptor EP4

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 3 µg/ml.

ターゲット情報

画像

  • ICC/IF image of ab92763 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab92763 has not yet been referenced specifically in any publications.

レビューと Q&A

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The peptide sequence used to raise the anti-Prostaglandin E Receptor EP4 antibody (ab92763) is SLRTQDATQTSCSTQSDASKQADL and the sequence for the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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