SureLight® APC Anti-PGE2 receptor EP4 subtype 抗体 (ab92763)
Key features and details
- SureLight® APC Rabbit polyclonal to PGE2 receptor EP4 subtype
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: SureLight® APC. Ex: 652nm, Em: 657nm
- Isotype: IgG
製品の概要
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製品名
SureLight® APC Anti-PGE2 receptor EP4 subtype antibody
PGE2 receptor EP4 subtype 一次抗体 製品一覧 -
製品の詳細
SureLight® APC Rabbit polyclonal to PGE2 receptor EP4 subtype -
由来種
Rabbit -
標識
SureLight® APC. Ex: 652nm, Em: 657nm -
特異性
ab92763 is reactive with EP4 receptor and non-reactive with EP1, EP2, and EP3 receptors. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Cow, Chimpanzee, Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to Human PGE2 receptor EP4 subtype aa 459-488 (C terminal).
Sequence:GSGRAGPAPKGSSLQVTFPSETLNLSEKCI
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.58% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92763の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. -
組織特異性
High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 450195 Chimpanzee
- Entrez Gene: 282331 Cow
- Entrez Gene: 5734 Human
- Entrez Gene: 100009081 Rabbit
- Omim: 601586 Human
- SwissProt: Q95KZ0 Chimpanzee
- SwissProt: Q8MJ08 Cow
- SwissProt: P35408 Human
see all -
別名
- EP 4 antibody
- EP4 antibody
- EP4R antibody
see all
画像
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ICC/IF image of ab92763 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab92763 は 3 報の論文で使用されています。
- Xue P et al. PGE2/EP4 skeleton interoception activity reduces vertebral endplate porosity and spinal pain with low-dose celecoxib. Bone Res 9:36 (2021). PubMed: 34334792
- Chen H et al. Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis. Nat Commun 10:181 (2019). PubMed: 30643142
- Ni S et al. Sensory innervation in porous endplates by Netrin-1 from osteoclasts mediates PGE2-induced spinal hypersensitivity in mice. Nat Commun 10:5643 (2019). PubMed: 31822662