Anti-Prostaglandin E Receptor EP4 抗体 (ab45863)

製品の概要

  • 製品名
    Anti-Prostaglandin E Receptor EP4 antibody
    Prostaglandin E Receptor EP4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Prostaglandin E Receptor EP4
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Guinea pig, Dog, Chimpanzee, Baboon, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    C-LEREVSKNPDLQA

    , corresponding to Internal sequence amino acids 302-314 of Human Prostaglandin E Receptor EP4

  • ポジティブ・コントロール
    • Human Testes lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    Peptide ELISA: Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
    • 組織特異性
      High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
    • 配列類似性
      Belongs to the G-protein coupled receptor 1 family.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EP 4 antibody
      • EP4 antibody
      • EP4R antibody
      • MGC126583 antibody
      • PE2R4_HUMAN antibody
      • PGE receptor EP4 subtype antibody
      • PGE2 receptor EP4 subtype antibody
      • Prostaglandin E receptor 4 antibody
      • Prostaglandin E receptor 4 subtype EP4 antibody
      • Prostaglandin E2 receptor antibody
      • Prostaglandin E2 receptor EP4 subtype antibody
      • Prostanoid EP4 receptor antibody
      • PTGER 2 antibody
      • PTGER 4 antibody
      • PTGER2 antibody
      • PTGER4 antibody
      see all

    画像

    • Anti-Prostaglandin E Receptor EP4 antibody (ab45863) at 0.1 µg/ml + Human Testes lysate (35µg protein in RIPA buffer)

      Predicted band size : 53 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab45863 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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