Anti-Prostaglandin E Receptor EP4 抗体 (ab45295)
製品の概要
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製品名Anti-Prostaglandin E Receptor EP4 antibody
Prostaglandin E Receptor EP4 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Prostaglandin E Receptor EP4
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rat, Chimpanzee
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Prostaglandin E Receptor EP4.
(Peptide available as ab45294.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: Raji and Ramos
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab45295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 53 kDa). |
ターゲット情報
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機能Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
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組織特異性High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
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配列類似性Belongs to the G-protein coupled receptor 1 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 450195 Chimpanzee
- Entrez Gene: 5734 Human
- Entrez Gene: 84023 Rat
- Omim: 601586 Human
- SwissProt: Q95KZ0 Chimpanzee
- SwissProt: P35408 Human
- SwissProt: P43114 Rat
- Unigene: 199248 Human
see all -
別名
- EP 4 antibody
- EP4 antibody
- EP4R antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin E Receptor EP4 antibody (ab45295)ICC/IF image of ab45295 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45295, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (5 min) HeLa cells at 5µg/ml. -
Western blot - Anti-Prostaglandin E Receptor EP4 antibody (ab45295)Anti-Prostaglandin E Receptor EP4 antibody (ab45295) at 1 µg/ml + Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 73 kDa (why is the actual band size different from the predicted?)
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab45295 detects a 73 kDa band in Western Blot which we believe corresponds to the glycosylated form of the protein.
参考文献
This product has been referenced in:
- Wang J et al. Activation of PGE2/EP2 and PGE2/EP4 signaling pathways positively regulate the level of PD-1 in infiltrating CD8+T cells in patients with lung cancer. Oncol Lett 15:552-558 (2018). Read more (PubMed: 29285200) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"