Anti-Prostaglandin E Receptor EP1 抗体 (ab183073)

製品の概要

  • 製品名
    Anti-Prostaglandin E Receptor EP1 antibody
    Prostaglandin E Receptor EP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Prostaglandin E Receptor EP1
  • 特異性
    ab183073 detects endogenous level of total Prostaglandin E Receptor EP1 protein.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide within Human Prostaglandin E Receptor EP1 aa 399-402. The exact sequence is proprietary. Genbank: NP_000946
    Sequence:

    LSHF


    Database link: P34995

  • ポジティブ・コントロール
    • 293T cell lysate, Human lymphoma tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab183073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/450 - 1/5000. Predicted molecular weight: 42 kDa.

ターゲット情報

  • 関連性
    PTGER1 is a subtype 1 receptor for prostaglandin E2 (PGE2). This receptor is coupled to the phosphatidylinositol-calcium second messenger system by G(q) proteins. PTGER1 may be an important modulator of renal function and is implicated in the smooth muscle contractile response to PGE2 in various tissues.
  • 細胞内局在
    Cell Membrane; multi-pass membrane protein.
  • 参照データベース
  • 別名
    • EP1 antibody
    • PGE receptor EP1 subtype antibody
    • PGE2 receptor EP1 subtype antibody
    • Prostaglandin E receptor 1 subtype EP1 42kDa antibody
    • Prostaglandin E receptor 1 subtype EP1 antibody
    • Prostaglandin E2 receptor EP1 subtype antibody
    • Prostanoid EP1 receptor antibody
    • PTGER1 antibody
    see all

画像

  • All lanes : Anti-Prostaglandin E Receptor EP1 antibody (ab183073) at 1/450 dilution

    Lane 1 : Human lymphoma tissue lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 42 kDa


    Exposure time : 1 minute

    10%SDS-PAGE

プロトコール

参考文献

ab183073 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Pooled endometrial tissue)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Pooled endometrial tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録