製品の概要

  • 製品名Anti-Prostaglandin dehydrogenase 1 antibody
    Prostaglandin dehydrogenase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Prostaglandin dehydrogenase 1
  • 特異性This antibody is specific for Prostaglandin dehydrogenase 1.
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Purified human placental prostaglandin dehydrogenase 1.

  • ポジティブ・コントロール
    • A549 lung carcinoma cell lysates treated with IL-6, DHT, and forskolin. LoVo colon carcinoma cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
IHC-P
  • 追加情報IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: 1/500 - 1/10000. Detects a bands of approximately 29 kDa (active form) and 58 kDa (predicted molecular weight: 29 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.
    • 組織特異性Detected in colon epithelium (at protein level).
    • 関連疾患Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) [MIM:259100]; also known as pachydermoperiostosis autosomal recessive. Primary hypertrophic osteoarthropathy is characterized by digital clubbing, osterarthropathy, variable features of pachydermia, delayed closure of the fontanels, and congenital heart disease.
      Defects in HPGD are the cause of cranioosteoarthropathy (COA) [MIM:259100]. Clinical features include infantile onset of swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature.
      Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC) [MIM:119900]; also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved.
    • 配列類似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 15 hydroxyprostaglandin dehydrogenase [NAD+] antibody
      • 15 PGDH antibody
      • 15-hydroxyprostaglandin dehydrogenase [NAD+] antibody
      • 15-PGDH antibody
      • 15PGDH antibody
      • Hpgd antibody
      • Hydroxyprostaglandin dehydrogenase 15 (NAD) antibody
      • NAD+ dependent 15 hydroxyprostaglandin dehydrogenase antibody
      • OTTHUMP00000218960 antibody
      • OTTHUMP00000219016 antibody
      • OTTHUMP00000219018 antibody
      • PGDH antibody
      • PGDH_HUMAN antibody
      • PGDH1 antibody
      • PHOAR1 antibody
      • Prostaglandin dehydrogenase 1 antibody
      • SDR36C1 antibody
      • Short chain dehydrogenase/reductase family 36C member 1 antibody
      see all

    Anti-Prostaglandin dehydrogenase 1 antibody 画像

    • Lane 1 : Anti-Prostaglandin dehydrogenase 1 antibody (ab37148) at 1/6000 dilution
      Lane 2 : Anti-Prostaglandin dehydrogenase 1 antibody (ab37148) at 1/500 dilution

      Lane 1 : LoVo whole cell lysate
      Lane 2 : LoVo whole cell lysate

      Developed using the ECL technique

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
      Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 seconds

    Anti-Prostaglandin dehydrogenase 1 antibody (ab37148) 使用論文

    This product has been referenced in:
    • Miyoshi J  et al. 5-aminosalicylic acid mediates expression of cyclooxygenase-2 and 15-hydroxyprostaglandin dehydrogenase to suppress colorectal tumorigenesis. Anticancer Res 32:1193-202 (2012). WB . Read more (PubMed: 22493349) »
    • Roberts HR  et al. Colon tumour cells increase PGE2 by regulating COX-2 and 15-PGDH to promote survival during the microenvironmental stress of glucose deprivation. Carcinogenesis 32:1741-7 (2011). WB ; Human . Read more (PubMed: 21926111) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Lung Tumors)
    Loading amount 35 µg
    Specification Lung Tumors
    Gel Running Conditions Reduced Denaturing
    Blocking step Li-Cor Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 17 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"