製品の概要

  • 製品名Anti-ProSAAS antibody
    ProSAAS 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ProSAAS
  • アプリケーション適用あり: WB, IHC-P, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 173-261 of Human PCSK1N with proprietary 26 kDa tag (NP_037403).

  • ポジティブ・コントロール
    • PCSK1N transfected 293T cell line lysate; Human cerebellum tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能May function in the control of the neuroendocrine secretory pathway. Proposed be a specific endogenous inhibitor of PCSK1. ProSAAS and Big PEN-LEN, both containing the C-terminal inhibitory domain, but not the further processed peptides reduce PCSK1 activity in the endoplasmic reticulum and Golgi. It reduces the activity of the 84 kDa form but not the autocatalytically derived 66 kDa form of PCSK1. Subsequent processing of proSAAS may eliminate the inhibition. Slows down convertase-mediated processing of proopiomelanocortin and proenkephalin. May control the intracellular timing of PCSK1 rather than its total level of activity. The function of the processed secreted peptides is not known.
  • 組織特異性Expressed in brain and pancreas.
  • ドメインProSAAS(1-180) increases secretion of enzymatically inactive PCSK1.
    The C-terminal inhibitory domain is involved in inhibtion of PCSK1. It corresponds to the probable processing intermediate Big PEN-LEN, binds to PCSK1 in vitro and contains the hexapeptide L-L-R-V-K-R, which, as a synthetic peptide, is sufficient for PCSK1 inhibition.
  • 翻訳後修飾Proteolytically cleaved in the Golgi.
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Secreted. Golgi apparatus > trans-Golgi network. A N-terminal processed peptide, probably Big SAAS or Little SAAS, is accumulated in cytoplasmic protein tau deposits in frontotemporal dementia and parkinsonism linked to chromosome 17 (Pick disease), Alzheimer disease and amyotrophic lateral sclerosis-parkinsonism/dementia complex 1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • b-LEN antibody
    • b-PEN-LEN antibody
    • b-SAAS antibody
    • Big LEN antibody
    • granin like neuroendocrine peptide antibody
    • l-LEN antibody
    • l-SAAS antibody
    • N-proSAAS antibody
    • OTTHUMP00000032426 antibody
    • PCSK1_HUMAN antibody
    • Pcsk1n antibody
    • pro-SAAS antibody
    • Proprotein convertase 1 inhibitor antibody
    • Proprotein convertase subtilisin/kexin type 1 inhibitor antibody
    • PROSAAS antibody
    • SAAS antibody
    • SAAS CT(1-49) antibody
    • SAAS CT(25-40) antibody
    see all

Anti-ProSAAS antibody 画像

  • Anti-ProSAAS antibody (ab118352) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 27 kDa
  • All lanes : Anti-ProSAAS antibody (ab118352) at 5 µg/ml

    Lane 1 : PCSK1N transfected 293T cell line lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 27 kDa
  • ab118352, at 3 µg/ml staining PCSK1N in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • Detection limit for ab118352 is 0.1 ng/ml as a capture antibody.

Anti-ProSAAS antibody (ab118352) 使用論文

ab118352 has not yet been referenced specifically in any publications.

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