製品の概要

  • 製品名Anti-Proprotein Convertase 2 antibody
    Proprotein Convertase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Proprotein Convertase 2
  • アプリケーション適用あり: ICC, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse Proprotein Convertase 2 aa 622-638.
    Sequence:

    ELDEAVERSLQSILRKN


    (Peptide available as ab5012)

  • ポジティブ・コントロール
    • WB: TC 6 cell extract.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab3533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/20 - 1/200.
ICC/IF 1/20 - 1/200.
WB Use a concentration of 2 µg/ml. Can be blocked with Proprotein Convertase 2 peptide (ab5012).

This antibody detects an ~65 kDa protein representing Proprotein 2 from alpha TC 6 cell extract.

ターゲット情報

Anti-Proprotein Convertase 2 antibody 画像

  • Anti-Proprotein Convertase 2 antibody (ab3533) at 1/500 dilution + Mouse cerebellum cell lysate at 25 µg

    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/Immunofluorescent analysis of Proprotein Convertase 2 (green) showing staining in the cytoplasm of HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescent analysis of Proprotein Convertase 2 (green) showing staining in the cytoplasm of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3533 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-Proprotein Convertase 2 antibody (ab3533) 使用論文

This product has been referenced in:
  • Gagnon J  et al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009). Read more (PubMed: 18706454) »

See 1 Publication for this product

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