製品の概要

  • 製品名
    Propidium Iodide
  • 製品の概要

    Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.

    For Microscopy analysis: PI can be viewed using rhodamine(red) filter (?= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).

    Flow Cytometry analysis: PI staining can be monitored in FL2 (DNA content) or FL3 (viability) channel.

    If used together as control for Annexin V assays ab14082, ab14083 or ab14152, PI should be diluted to 250 µg/ml solution (in PBS) prior use and added as 1 µl/ Annexin V Assay (0.25µg/assay).

    Visit our FAQs page for tips and troubleshooting.

  • アプリケーション
    適用あり: FMmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
FM Use at an assay dependent concentration.

画像

  • Vero-DogSLAM (VDS) (Vero- stably transfected with canine SLAM) cells and Vero (African Green Monkey kidney) cells were infected with wtCDV. Cells were fixed, permeabilised and stained with SSPE serum and rabbit anti-human FITC; nuclei were stained using ab14083 propidium iodide. Images were taken using a Nikon Eclipse TE2000-U UV microscope (x400). 

参考文献

This product has been referenced in:
  • Kheder S  et al. Effects of prolonged exposure to low dose metformin in thyroid cancer cell lines. J Cancer 8:1053-1061 (2017). Read more (PubMed: 28529619) »
  • Peng X  et al. Cytotoxic and apoptotic activities of black widow spiderling extract against HeLa cells. Exp Ther Med 13:3267-3274 (2017). Read more (PubMed: 28587399) »

See all 8 Publications for this product

レビューと Q&A

Here is a link to the PI staining protocol I found, http://www.abcam.com/protocols/flow-cytometric-analysis-of-cell-cycle-with-propidium-iodide-dna-staining


It suggests using a solution of 50ug/mL, so dilute the amount of the 1mg/mL stock...

Read More

I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cy...

Read More

Thank you for your reply.

Both Annexin V-EGFP Apoptosis Detection Reagents (ab14152 and ab14082) contain a protocol booklet in their datasheets. These are the recommended protocols to use along with the Propidium Iodide reagent.

Ple...

Read More

rr/>

I confirm the product Propiduim Iodide ab14083 can definitely be used with paraffin-embedded, formalin-fixed tissues.

Thank you for your comprehension, and please do not hesitate to contact us back for further assistance.

Thank you for contacting us.

Yes, Propidium Iodide ab14083 can definitely be used with fixed tissue samples.

Please do not hesitate to contact us if you need any more advice or information.

Vielen Dank für Ihre Geduld.

Wie versprochen habe ich mich nach den aktuellen Details des Propidiumiodid-Staining Protokolls erkundigt – an diesem seit Jahren bewährten Protokoll hat sich nichts geändert. Ein Mitarbeiter ...

Read More

Thank you for sending your data, it was very helpful in this case. Since the first peak is much higher, I suspect it should be a combination of sub G1 and cell debris. Usually if it is cell debris, it should just be at the bottom and there shouldn’t be...

Read More

Thank you for calling Abcam earlier. I am going to email the lab about your query and I just wanted to make sure I had written it down correctly, were you asking: "Will Propidium Iodide label the sub G0 phase" Is that correct? If so, I will go ahead an...

Read More

Thank you for your enquiry and your patience. I do not have any specific advice on why you see staining in the yolk. The best resource is probably the protocols you can find in the zebrafish book at www.zfin.org I believe that methanol washes ma...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録