製品の概要

  • 製品名
    Anti-Prolyl Endopeptidase antibody
    Prolyl Endopeptidase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Prolyl Endopeptidase
  • 特異性
    ab58988 does not recognize other Prolyl Endopeptidase family members.
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide based on the amino terminal end of human Prolyl Endopeptidase. (Peptide available as ab63973.) Immunizing peptide available on request, please contact a customer service representative to ask for availability.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-Fr
  • 追加情報
    WB: 1/1000 - 1/5000 dependent on substrate. Predicted molecular weight: 81 kDa.
    A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.

    EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    • All lanes : Anti-Prolyl Endopeptidase antibody (ab58988) at 1/1000 dilution

      Lane 1 : Madin Darby canine kidney (MDCK) cell lysate at 10 µl
      Lane 2 : Madin Darby canine kidney (MDCK) cell lysate at 5 µl


      Predicted band size : 81 kDa

    参考文献

    This product has been referenced in:
    • Shinde MY  et al. Phosphoproteomics reveals that glycogen synthase kinase-3 phosphorylates multiple splicing factors and is associated with alternative splicing. J Biol Chem 292:18240-18255 (2017). WB ; Mouse . Read more (PubMed: 28916722) »
    • Justa-Schuch D  et al. The amino terminus extension in the long dipeptidyl peptidase 9 isoform contains a nuclear localization signal targeting the active peptidase to the nucleus. Cell Mol Life Sci 71:3611-26 (2014). WB . Read more (PubMed: 24562348) »

    See all 6 Publications for this product

    レビューと Q&A

    Thank you for contacting us.

    Based on a 95% homology between the bovine and human forms of the protein, each of the three antibodies should cross react with bovine prolyl endopeptidase. As none of these product has been tested in bovine howe...

    Read More

    Thank you for contacting Abcam. The immunogen sequence for ab58988 is considered to be proprietary and so I am unable to access the information that you require. I have looked through all of the other Prolyl Endopeptidase antibodies in our catalogue an...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 25°C
    Username

    Mrs. Lisa Bruce

    Verified customer

    投稿 Sep 26 2011

    Thank you for contacting us. Please find a protocol attached for performing heat-mediated antigen retrieval in free floating sections (PMID: 10634500). Additionally, the following website provides a protocol for performing heat-mediated antigen...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録