Anti-Prolyl Endopeptidase 抗体 (ab110857)

製品の概要

  • 製品名Anti-Prolyl Endopeptidase antibody
    Prolyl Endopeptidase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Prolyl Endopeptidase
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-QYIVGRSRKQSNP

    , corresponding to internal sequence amino acids 657-669 of Human Prolyl Endopeptidase.

  • ポジティブ・コントロール
    • Human kidney and tonsil tissues.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99.48% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab110857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

Anti-Prolyl Endopeptidase antibody 画像

  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

Anti-Prolyl Endopeptidase antibody (ab110857) 使用論文

ab110857 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

投稿 Oct 23 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Rat Tissue sections (kidney)
Specification kidney
Permeabilization Yes - TritonX-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 19 2014

Thank you for contacting us. Please find a protocol attached for performing heat-mediated antigen retrieval in free floating sections (PMID: 10634500). Additionally, the following website provides a protocol for performing heat-mediated antigen...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"