Anti-Prolyl Endopeptidase 抗体 (ab110857)

製品の概要

  • 製品名
    Anti-Prolyl Endopeptidase antibody
    Prolyl Endopeptidase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Prolyl Endopeptidase
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-QYIVGRSRKQSNP

    , corresponding to internal sequence amino acids 657-669 of Human Prolyl Endopeptidase.

  • ポジティブ・コントロール
    • Human kidney and tonsil tissues.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99.48% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

Anti-Prolyl Endopeptidase antibody 画像

  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

プロトコール

Anti-Prolyl Endopeptidase antibody (ab110857) 使用論文

ab110857 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
10% buffered formalin
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投稿 Oct 23 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Sample
Rat Tissue sections (kidney)
Specification
kidney
Permeabilization
Yes - TritonX-100
Fixative
Formaldehyde
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投稿 Mar 19 2014

Thank you for contacting us. Please find a protocol attached for performing heat-mediated antigen retrieval in free floating sections (PMID: 10634500). Additionally, the following website provides a protocol for performing heat-mediated antigen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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