製品の概要
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製品名Anti-Prolactin Receptor antibody [T6]
Prolactin Receptor 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [T6] to Prolactin Receptor
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特異性Detects prolactin (PRL) receptor in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.
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アプリケーション適用あり: ICC, IHC-Fr, WB, IP, Flow Cyt, Inhibition Assay, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Sheep, Human
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免疫原
Other Immunogen Type corresponding to Rat Prolactin Receptor. Purified rat liver PRL receptor.
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ポジティブ･コントロール
- WB and ICC: NB2 cells
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Concentration information loading... -
精製度IgG fraction
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一次抗体 備考Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
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ポリ/モノモノクローナル
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クローン名T6
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アイソタイプIgG2a
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab2773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use a concentration of 5 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| Inhibition Assay | Use at an assay dependent concentration. | |
| ICC/IF | 1/200. |
ターゲット情報
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機能This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
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組織特異性Expressed in breast, placenta, kidney, liver and pancreas.
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配列類似性Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains. -
ドメインThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
細胞内局在Secreted and Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 5618 Human
- Entrez Gene: 19116 Mouse
- Entrez Gene: 24684 Rat
- Omim: 176761 Human
- SwissProt: P16471 Human
- SwissProt: Q08501 Mouse
- SwissProt: P05710 Rat
- Unigene: 368587 Human
see all -
別名
- AI987712 antibody
- CLONE SPM213 antibody
- CPRLP antibody
see all
Anti-Prolactin Receptor antibody [T6] 画像
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![Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor [T6] antibody (ab2773)](http://a.abcam.com/ps/products/2/ab2773/Images/ab2773-219012-ab2773iccif3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor [T6] antibody (ab2773)Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor [T6] antibody (ab2773)](http://a.abcam.com/ps/products/2/ab2773/Images/ab2773-219011-ab2773iccif2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor [T6] antibody (ab2773)Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in H-4-II-E cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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![Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor [T6] antibody (ab2773)](http://a.abcam.com/ps/products/2/ab2773/Images/ab2773-219010-ab2773iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Prolactin Receptor [T6] antibody (ab2773)Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in SW480 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor [T6] antibody (ab2773)](http://a.abcam.com/ps/products/2/ab2773/Images/ab2773-219009-ab2773ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prolactin Receptor [T6] antibody (ab2773)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized rat pituitary gland tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
プロトコール
Anti-Prolactin Receptor antibody [T6] (ab2773) 使用論文
This product has been referenced in:
- Dardenne M et al. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endocrinology 134:2108-14 (1994). Read more (PubMed: 8156910) »
- Rui H et al. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology 135:1299-306 (1994). Read more (PubMed: 7925093) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"