製品の概要

  • 製品名Anti-Proinsulin antibody [C-PEP-01]
    Proinsulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [C-PEP-01] to Proinsulin
  • アプリケーション適用あり: ELISA, IHC-P, RIA, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    C-peptide (Human) conjugated to BSA.

  • 特記事項


    The antibody can be used for RIA and ELISA estimation of human proinsulin C-peptide or human proinsulin in body fluids.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites using precipitation methods.
  • 一次抗体 備考The antibody can be used for RIA and ELISA estimation of human proinsulin C-peptide or human proinsulin in body fluids.
  • ポリ/モノモノクローナル
  • クローン名C-PEP-01
  • アイソタイプIgG1
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab7761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P 1/200 - 1/500.
RIA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 関連性A precursor of insulin it is converted to insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • INS antibody
    • insulin antibody
    • IRDN antibody
    • Proinsulin precursor antibody

Anti-Proinsulin antibody [C-PEP-01] 画像

  • Ab7761 staining human normal pancreas tissue. Staining is localised to extracellular space.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Proinsulin antibody [C-PEP-01] (ab7761) 使用論文

This product has been referenced in:
  • Hilgert I  et al. A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA. Hybridoma 10:379-86 (1991). Read more (PubMed: 1916848) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This antibody is very specific and does not cross-react with active insulin. Please do not hesitate to contact us if you have additional questions.

Thank you for your enquiry regarding ab12762(25µg), ab12764(25µg), ab12769(25µg), ab11022(1ml), ab10988(200µl) and ab7761(100µg). These products are all priced at $295 US dollars. In the future you can view pricing,availability and detailed information...

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This antibody has only been tested on human pro-insulin. You will have to compare the sequences of the rat and human to determine whether you believe there may be some cross-reactivity.

This antibody works well in ELISA, but we have no data yet for its use on rat and hamster tissues.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"