製品の概要

  • 製品名Anti-Proinsulin antibody [3A1]
    Proinsulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3A1] to Proinsulin
  • 特異性Kd for this antibody is >1.1 x 10-8M This antibody is specific for proinsulin. It does not recognise insulin or C-peptide.
  • アプリケーション適用あり: ICC/IF, ELISA, IHC-Fr, RIAmore details
  • 種交差性
    交差種: Human
    非交差種: Cow, Pig
  • 免疫原

    Full length native protein (purified) (Human).

  • エピトープThis antibody reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

ターゲット情報

  • 関連性A precursor of insulin it is converted to insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • INS antibody
    • insulin antibody
    • IRDN antibody
    • Proinsulin precursor antibody

Anti-Proinsulin antibody [3A1] 画像

  • ab8301 at a 1/500 dilution staining human pancreas tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 1 hour and then detected using an HRP conjugated system.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.

    See Abreview

Anti-Proinsulin antibody [3A1] (ab8301) 使用論文

This product has been referenced in:
  • Cao M  et al. PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance. PLoS Biol 11:e1001541 (2013). Read more (PubMed: 23630453) »
  • Colombo C  et al. Seven mutations in the human insulin gene linked to permanent neonatal/infancy-onset diabetes mellitus. J Clin Invest : (2008). ICC/IF ; Human . Read more (PubMed: 18451997) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Permeabilization Yes - citrate buffer
Specification Brain
Blocking step 10% Methanol+3% H2O2 in PBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
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投稿 Sep 23 2015

Vielen Dank für Ihre Anfrage. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 auch nicht mit Mausproben im ELISA funktioniert. Wenn ich mich richtig erinnere, hatten wir am Telefon jedoch auch besprochen, dass ab8301 bisher nicht in der Mau...

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Vielen Dank für diese Rückmeldung. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 nicht optimal für Ihre Zwecke geeignet ist. Leider ist eine nur sehr geringe Verdünnung keiner der Gründe, bei dem es mir erlaubt ist, einen kostenlosen Ersa...

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Thank you for your inquiry. Ab8301 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule. According to our info, ab8301 has been tested only with unprocessed proinsulin. I hope that this information is helpfu...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (frozen and FFPE pancreas)
Specification frozen and FFPE pancreas
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
Username

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Verified customer

投稿 Dec 12 2005

Clone 3A1 (ab8301) reacts with the site of connection of C-peptide and C-end of B-chain of the proinsulin molecule. Clones 1D6 (ab8299) and 1D3 (ab8300) react with different epitopes on the human proinsulin molecule, both of each are generated by the ...

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These are some of our newest products and all the information we have on each of them is featured on the datasheets. As yet, there are no published references. Pricing information in GBP, EUR and USD can be found by clicking on the "prices and buying" ...

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The antibody has not yet been tested on paraffin embedded sections, although it has been shown to be suitable for use in IHC with frozen sections. It has not been tested on mouse although we note that it does not cross-react with bovine or porcine proi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"