製品の概要

  • 製品名
    Anti-Proinsulin antibody [3A1]
  • 製品の詳細
    Mouse monoclonal [3A1] to Proinsulin
  • 由来種
    Mouse
  • 特異性
    Kd for this antibody is >1.1 x 10-8M This antibody is specific for proinsulin. It does not recognise insulin or C-peptide.
  • アプリケーション
    適用あり: ICC/IF, ELISA, IHC-Fr, RIAmore details
  • 種交差性
    交差種: Human
    非交差種: Cow, Pig
  • 免疫原

    Full length native protein (purified) (Human).

  • エピトープ
    This antibody reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    A precursor of insulin it is converted to insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced.
  • 細胞内局在
    Secreted
  • 参照データベース
    • 別名
      • INS antibody
      • insulin antibody
      • IRDN antibody
      • Proinsulin precursor antibody

    画像

    • Immunohistochemistry (Frozen sections) - Anti-Proinsulin antibody [3A1] (ab8301)
      Immunohistochemistry (Frozen sections) - Anti-Proinsulin antibody [3A1] (ab8301)

      ab8301 at a 1/500 dilution staining human pancreas tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 1 hour and then detected using an HRP conjugated system.

      This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.

      See Abreview

    参考文献

    This product has been referenced in:
    • Matsunaga K  et al. Rab2a and Rab27a cooperatively regulate the transition from granule maturation to exocytosis through the dual effector Noc2. J Cell Sci 130:541-550 (2017). Read more (PubMed: 27927751) »
    • Cao M  et al. PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance. PLoS Biol 11:e1001541 (2013). Read more (PubMed: 23630453) »

    See all 4 Publications for this product

    ab8301 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    8 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Frozen sections) abreview for Anti-Proinsulin antibody [3A1]

     Good
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - citrate buffer
    Specification
    Brain
    Blocking step
    10% Methanol+3% H2O2 in PBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Sep 23 2015

    Leider hat sich bei meinen Testungen herraus gestellt, das der Proinsulin (ab8301) Antikörper Elisa mit unseren Maus Proben nicht funktioniert. Ich würde deshalb gerne nochmal einen anderen ausprobieren wollen. Es wäre sehr nett von Ihnen, wenn Sie mir...

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    Vielen Dank für Ihre Anfrage. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 auch nicht mit Mausproben im ELISA funktioniert. Wenn ich mich richtig erinnere, hatten wir am Telefon jedoch auch besprochen, dass ab8301 bisher nicht in der Mau...

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    Leider hat sich bei meinen Testungen herraus gestellt, das ich für den Eilsa mit unseren Maus Proben den Proinsulin (ab8301) Antikörper nur sehr gering verdünnen kann. Ich würde deshalb gerne nochmal einen anderen ausprobieren wollen. Es wäre sehr nett...

    Read More

    Vielen Dank für diese Rückmeldung. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 nicht optimal für Ihre Zwecke geeignet ist. Leider ist eine nur sehr geringe Verdünnung keiner der Gründe, bei dem es mir erlaubt ist, einen kostenlosen Ersa...

    Read More

    I have a question regarding Proinsulin antibody [3A1] (ab8301), is it suitable for staining of proinsulin I or II or both?

    Thank you for your inquiry. Ab8301 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule. According to our info, ab8301 has been tested only with unprocessed proinsulin. I hope that this information is helpfu...

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-Proinsulin antibody [3A1]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (frozen and FFPE pancreas)
    Specification
    frozen and FFPE pancreas
    Fixative
    Formaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Dec 12 2005

    Customer is interested in these 3 proinsulin antibodies (ab8299, ab8300, ab8301). Were they raised against different sequences? How exactly do they differ (other than being different clones)?

    Clone 3A1 (ab8301) reacts with the site of connection of C-peptide and C-end of B-chain of the proinsulin molecule. Clones 1D6 (ab8299) and 1D3 (ab8300) react with different epitopes on the human proinsulin molecule, both of each are generated by the ...

    Read More

    Please send literature refs for ab8301, ab8300 and ab8299. What do you know about the antigenic sites for these antibodies? Also send price quotes. Thanks

    These are some of our newest products and all the information we have on each of them is featured on the datasheets. As yet, there are no published references. Pricing information in GBP, EUR and USD can be found by clicking on the "prices and buying" ...

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    will this work on formalin fixed paraffin embedded tissues? Any cross reactivity with mouse? Also, I'm looking for MIB1, any suggestions? Karen Mundwiler

    The antibody has not yet been tested on paraffin embedded sections, although it has been shown to be suitable for use in IHC with frozen sections. It has not been tested on mouse although we note that it does not cross-react with bovine or porcine proi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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