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BALB/C mice were injected with purified recombinant rat prohibitin protein.
Our Abpromise guarantee covers the use of ab1836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.|
|IHC-Fr||1/50 - 1/100. ABC method.|
|IP||Use at an assay dependent concentration.|
|WB||1/100. (see Abreview).|
|Flow Cyt||Use 1µg for 106 cells.|
ab1836 at a 1/100 dilution staining mouse Cardiac muscle tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the antibody was incubated with the tissue for 12 hours. Bound antibody was detected with a HRP conjuaged anti-mouse antibody.
The picture shown is a transverse section of mouse ventricular wall; from TEM studies the distribution of mitochondria among the myofibrils is well-established, and so in this 2-um-thick section there exists both minimal superposition as well as a staining pattern which is consistent with what is expected for cardiac mitochondrial patterns (arrows in the inset indicate groups of prohibitin-positive mitochondria).
This image is courtesy of an Abreview submitted by Mike Forbes on 29 March 2006.