The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 30 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Widely expressed in different tissues.
Belongs to the prohibitin family.
Levels of expression in fibroblasts decrease heterogeneously during cellular aging.