製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Prohibitin
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to near C terminus of human Prohibitin protein.

  • ポジティブ・コントロール
    • Human heart.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報
    IHC-P: 1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    WB: Use at an assay dependent dilution. Predicted molecular weight: 30 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
    • 組織特異性
      Widely expressed in different tissues.
    • 配列類似性
      Belongs to the prohibitin family.
    • 発生段階
      Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
    • 細胞内局在
      Mitochondrion inner membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Epididymis luminal protein 215 antibody
      • Epididymis secretory sperm binding protein Li 54e antibody
      • HEL 215 antibody
      • HEL S 54e antibody
      • PHB antibody
      • PHB_HUMAN antibody
      • PHB1 antibody
      • Prohibitin antibody
      see all

    画像

    • ab70672, at 1/100 dilution, staining Prohibitin in formalin fixed, paraffin embedded human heart tissue by immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Zhu X  et al. Long-circulating siRNA nanoparticles for validating Prohibitin1-targeted non-small cell lung cancer treatment. Proc Natl Acad Sci U S A 112:7779-84 (2015). Read more (PubMed: 26056316) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you very much for your patience.

    I have hear back now from the laboratories in regards to the potential crossreaction of the prohibitin antibodies with T. brucei. Unfortunately none of these antibodies seems to be likely to crossreact w...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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