Anti-Progesterone Receptor (phospho S554) 抗体 (ab101610)

製品の概要

• 製品名
  Anti-Progesterone Receptor (phospho S554) antibody
  Progesterone Receptor 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to Progesterone Receptor (phospho S554)
• アプリケーション
  適用あり: WBmore details
• 種交差性
  交差種: Human
  
• 免疫原

  Synthetic phosphopeptide, corresponding to amino acid residues surrounding S554 (within amino acids 549-561) of Human Progesterone Receptor.

• ポジティブ･コントロール
  • IMR32

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• バッファー
  Preservative: 0.01% Thimerosal (merthiolate)
  Constituents: 20% Glycerol, 1% BSA, 1 x PBS, pH 7.0
• Concentration information loading...
• 精製度
  Immunogen affinity purified
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgG
• 研究分野
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Progesterone

  • Neuroscience
  • Endocrine system
  • Gonadotrophic axis

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Progesterone

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Progesterone

  • Cancer
  • Tumor biomarkers
  • Receptors

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

アプリケーション

ターゲット情報

• 機能
  The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
  Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
  Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
• 配列類似性
  Belongs to the nuclear hormone receptor family. NR3 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• ドメイン
  Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
• 翻訳後修飾
  Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
  Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
  Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
  Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
• 細胞内局在
  Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.

• Target information above from: UniProt accession P06401 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 5241 Human
  • Omim: 607311 Human
  • SwissProt: P06401 Human
  • Unigene: 32405 Human
  • Unigene: 742403 Human
• 別名
  • NR3C3 antibody
  • Nuclear receptor subfamily 3 group C member 3 antibody
  • PGR antibody

  • PR antibody
  • PRA antibody
  • PRB antibody
  • PRGR_HUMAN antibody
  • Progesterone receptor antibody
  • Progestin receptor form A antibody
  • Progestin receptor form B antibody

  see all

プロトコール

• Western blot protocols