リコンビナント
RabMAb

Anti-Progesterone Receptor (phospho S190) 抗体 [EP1516Y] (ab75857)

製品の概要

  • 製品名
    Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y]
    Progesterone Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1516Y] to Progesterone Receptor (phospho S190)
  • 特異性
    ab75857 detects Progesterone Receptor phophorylated at serine 190.
  • アプリケーション
    適用あり: WB, IP, ICC, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 190 of human Progesterone Receptor

  • ポジティブ・コントロール
    • T47D cell lysates, untreated or treated with progesterone; human breast carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
IP 1/50.
ICC 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
      Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
      Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
    • 配列類似性
      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • ドメイン
      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 翻訳後修飾
      Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
      Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
      Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • 細胞内局在
      Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    画像

    • ab75857 at 1/100 dilution staining Progesterone Receptor in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857) at 1/10000 dilution

      Lane 1 : T47D cell lysates, untreated
      Lane 2 : T47D cell lysates, treated with progesterone

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 99 kDa
      Observed band size : 99,118 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Harigopal M  et al. Multiplexed assessment of the Southwest Oncology Group-directed Intergroup Breast Cancer Trial S9313 by AQUA shows that both high and low levels of HER2 are associated with poor outcome. Am J Pathol 176:1639-47 (2010). Read more (PubMed: 20150438) »

    See 1 Publication for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab75857.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録