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Our Abpromise guarantee covers the use of ab16661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100. PubMed: 18617611|
|WB||1/200. Predicted molecular weight: 99,120 kDa.|
|IHC-P||1/100. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.|
|Flow Cyt||1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.
ab16661 staining rat ovary tissue sections by IHC-P. Sections were fixed in 10% buffered formalin and subjected to heat mediated antigen retrieval in 10mM citrate buffer pH 6.0 prior to blocking with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1/50 in TBS (with Tween) and incubated with the sample for 16 hours at 4°C. A HRP conjugated goat anti-rabbit was used as the secondary antibody.