Anti-Progesterone Receptor 抗体 [SP2] (ab16661)

製品の概要

  • 製品名Anti-Progesterone Receptor antibody [SP2]
    Progesterone Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP2] to Progesterone Receptor
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Rabbit
  • 免疫原

    Recombinant fragment:

    AAFPDFPLGPPPPLPPRATPSRPGEAAVTAAPASASVSSASSSGSTLECI LYKAEGAPPQQGPFAPPPCKAPGASGCLLPRDGLPSTSASAAAAGAAPAL YPALGLNGLPQLGYQ

    , corresponding to amino acids 412-526 of Human Progesterone Receptor.

  • エピトープAmino acids 412-526
  • ポジティブ・コントロール
    • Breast carcinomas

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100. PubMed: 18617611
WB 1/200. Predicted molecular weight: 99,120 kDa.
IHC-P 1/100. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 細胞内局在Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

Anti-Progesterone Receptor antibody [SP2] 画像

  • Immunohistochemistry analysis of human breast carcinoma tissue labelling SP2 with ab16661.

  • ab16661 staining rat ovary tissue sections by IHC-P.  Sections were fixed in 10% buffered formalin and subjected to heat mediated antigen retrieval in 10mM citrate buffer pH 6.0 prior to blocking with 5% serum for 20 minutes at 20°C.  The primary antibody was diluted 1/50 in TBS (with Tween) and incubated with the sample for 16 hours at 4°C.  A HRP conjugated goat anti-rabbit was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing T47D cells stained with ab16661 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16661, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Progesterone Receptor antibody [SP2] (ab16661) 使用論文

This product has been referenced in:
  • Samalecos A & Gellersen B Systematic expression analysis and antibody screening do not support the existence of naturally occurring progesterone receptor (PR)-C, PR-M, or other truncated PR isoforms. Endocrinology 149:5872-87 (2008). WB, ICC/IF ; Human . Read more (PubMed: 18617611) »
  • Huang Z et al. Development of new rabbit monoclonal antibody to progesterone receptor (clone SP2): No heat pretreatment but effective for paraffin section immunohistochemistry. Appl Immunohistochem Mol Morphol : (2005). Read more (PubMed: ) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 20% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

投稿 Jul 15 2013

Thank you for your enquiry.
I can confirm that ab16661 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the ...

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Thank you for your enquiry.

I can confirm that ab16661 Progesterone Receptor antibody SP2 is sold as tissue culture supernatant. TC supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determin...

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Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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Thank you for contacting us. This antibody has been tested on human breast carcinoma tissue sections in our own lab and as you can see from image it produced best results. One of the customers has used this antibody on Rat ovary tissue; as you can ...

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Thank you for contacting Abcam. Under our Abpromise we guarantee that ab16661 will work on rat tissue in IHC-P. As long as the protein is expressed in the ovarian tissues of the rat then this antibody will be able to detect it. If there...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat ovary, uterus)
Specification Rat ovary, uterus
Fixative 10% buffered formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"