Anti-Progesterone Receptor 抗体 [Alpha PR6] - ChIP Grade (ab2765)


  • 製品名
    Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade
    Progesterone Receptor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Alpha PR6] to Progesterone Receptor - ChIP Grade
  • 由来種
  • 特異性
    Detects the B form of the progesterone receptor (PR). This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.
  • アプリケーション
    適用あり: ICC, IHC-Fr, IP, WB, ChIP, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Human
  • 免疫原

    Other Immunogen Type corresponding to Chicken Progesterone Receptor. Progesterone receptor purified from chick oviduct cytosol.



Our Abpromise guarantee covers the use of ab2765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IHC-Fr Use a concentration of 20 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.
ChIP Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P 1/50.


  • 機能
    The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメイン
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾
    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • 細胞内局在
    Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on human uterus tissue. Antigen retrieval was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2765 (1:20) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.

  • Immunocytochemistry/ Immunofluorescence analysis of T47D cells untreated (left) or stimulated with 100nm promegestone for 1 hour (right), labeling Progesterone Receptor with ab2765 (green). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA for 15 minutes at room temperature. Cells were stained with Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at a dilution of 1/100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 goat anti-mouse IgG secondary antibody at a dilution of 1/1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.

  • All lanes : Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at 1 µg/ml

    Lane 1 : T47D cell lysate untreated (-)
    Lane 2 : T47D cell lysate stimulated (+) with 100 nm promegestone for 1 hour

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat anti-Mouse IgG-HRP at 1/2000 dilution

    Predicted band size: 99 kDa

  • ab2765 staining Progesterone Receptor in Rat mammary tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval by pressure cooker prior to blocking with 5% BSA for 5 minutes at room temperature. The primary antibody was diluted 1/100 in TBST and incubated with the sample for 16 hours at 4°C. A biotin-conjugated goat anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing T47D cells stained with ab2765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2765, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Bakrania AK  et al. Role of ß-Interferon Inducer (DEAE-Dextran) in Tumorigenesis by VEGF and NOTCH1 Inhibition along with Apoptosis Induction. Front Pharmacol 8:930 (2017). Read more (PubMed: 29311933) »
  • Cheang TY  et al. C14orf166 overexpression correlates with tumor progression and poor prognosis of breast cancer. J Transl Med 14:54 (2016). Read more (PubMed: 26883017) »

See all 12 Publications for this product

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Sep 01 2015

Vielen Dank für diese zusätzliche Information.

Leider haben wir keinen Antikörper den ich für die Detektion Ihres rekombinanten Proteins garantieren kann. Ich habe drei Antikörper gefunden die vielleicht für Sie i...

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Thank you for contacting us.

It will be 100ul.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Yes, the antibody is purified. The solution before lyophilization is 1X PBS with 0.05% sodium azide. I hope this is helpful. Please contact us again if you have any further questions.

Thank you for contacting us. I can confirm that the storage buffer for this antibody is PBS containing 0.05% sodium azide, and that it is purified from mouse ascites. Should I receive any further information from the lab, then I will let you know. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rat Tissue sections (mammary carcinoma)
mammary carcinoma
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pressure cooker
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C

孝子 赤松

Verified customer

投稿 Sep 27 2011

Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab2675 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event t...

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This sounds like a classic case of antibody storage condition problems. The antibody may be binding to the plastic tube as a result of the aliquot size being too small (i.e. less then 10ul). I would appreciate it if you could please tell me the am...

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I have just contacted the originator of this product. I would like to confirm that the there should always be extra protein in the tube so that it will help stabilize the antibody, especially for a monoclonal antibody. If you are going to use the antib...

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