製品の概要

  • 製品名
    Anti-Progesterone Receptor antibody
    Progesterone Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Progesterone Receptor
  • アプリケーション
    適用あり: IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to C terminal amino acids 412-562 of Human Progesterone Receptor

  • ポジティブ・コントロール
    • Breast Carcinomas

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
IHC-P
  • 追加情報
    IHC-Fr: Use at an assay dependent dilution (PMID 18505792).
    IHC-P: 1/200 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
      Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
      Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
    • 配列類似性
      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • ドメイン
      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 翻訳後修飾
      Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
      Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
      Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • 細胞内局在
      Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    画像

    • ab15509 staining Progesterone Receptor in breast carcinoma by Immunohistochemistry (FFPE-sections).

    プロトコール

    参考文献

    This product has been referenced in:
    • Guffanti E  et al. Nuclear pore complex proteins mark the implantation window in human endometrium. J Cell Sci 121:2037-45 (2008). IHC-Fr ; Human . Read more (PubMed: 18505792) »

    See 1 Publication for this product

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