製品の概要

  • 製品名Anti-Profilin 1 antibody [1D5]
    Profilin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1D5] to Profilin 1
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Rat, Dog, Human, Monkey
  • 免疫原

    Recombinant full length Human Profilin 1 produced in HEK293T cells (NP_005013).

  • ポジティブ・コントロール
    • HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cell extracts; Human Kidney and pancreas tissues; HeLa and Jurkat cells; COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1; HEK293T cell lysate transfected with pCMV6-ENTRY Profilin 1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Profilin 1 overexpress plasmid.
  • 特記事項Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 15 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
  • 配列類似性Belongs to the profilin family.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Actin binding protein antibody
    • ALS18 antibody
    • Epididymis tissue protein Li 184a antibody
    • OTTHUMP00000125244 antibody
    • PFN 1 antibody
    • Pfn antibody
    • PFN1 antibody
    • PROF1_HUMAN antibody
    • Profilin I antibody
    • Profilin-1 antibody
    • Profilin1 antibody
    • ProfilinI antibody
    see all

Anti-Profilin 1 antibody [1D5] 画像

  • All lanes : Anti-Profilin 1 antibody [1D5] (ab118984) at 1/1000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell lysate
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 15 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202338)
  • All lanes : Anti-Profilin 1 antibody [1D5] (ab118984) at 1/1000 dilution

    Lane 1 : HEK293T cell lysate transfected with the pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with the pCMV6-ENTRY Profilin 1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 15 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202338)
  • ab118984 at 1/50 dilution staining Profilin 1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab118984 at 1/50 dilution staining Profilin 1 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
  • ab118984 at 1/100 dilution staining Profilin 1 in COS7 cells transiently transfected with pCMV6-ENTRY Profilin 1 by Immunofluorescence.
  • ab118984 at 1/100 dilution staining Profilin 1 in HEK293T cells transfected with either pCMV6-ENTRY Profilin 1 overexpressing plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.
  • ab118984 at 1/100 dilution staining Profilin 1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab118984 at 1/100 dilution staining Profilin 1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Anti-Profilin 1 antibody [1D5] (ab118984) 使用論文

This product has been referenced in:
  • Ralhan R  et al. Immunohistochemical Subcellular Localization of Protein Biomarkers Distinguishes Benign from Malignant Thyroid Nodules: Potential for Fine-Needle Aspiration Biopsy Clinical Application. Thyroid 25:1224-34 (2015). IHC . Read more (PubMed: 26131603) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab118984.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"