製品の概要

  • 製品名
    Anti-ProDynorphin antibody
    ProDynorphin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ProDynorphin
  • 特異性
    This antibody blocks specifically with leumorphin (rat only). Cells are found in the paraventricular and supraoptic nucleus and fibres in the lateral hypothalamus in rat brain of colchicine treated 4% FA perfused tissue.
  • アプリケーション
    適用あり: IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    RSQENPNTYSEDLDV

    (Rat)

  • 特記事項
    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
    Whole serum is supplied - diluted.
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200 - 1/2000.

ターゲット情報

  • 機能
    Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.
    Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.
    Leumorphin has a typical opiod activity and may have anti-apoptotic effect.
  • 関連疾患
    Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23) [MIM:610245]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria.
  • 配列類似性
    Belongs to the opioid neuropeptide precursor family.
  • 翻訳後修飾
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADCA antibody
    • Alpha neoendorphin antibody
    • Beta neoendorphin antibody
    • Beta-neoendorphin-dynorphin antibody
    • Big Dyn antibody
    • Big dynorphin antibody
    • Dyn antibody
    • Dyn-A17 antibody
    • Dyn-B antibody
    • Dynorphin A antibody
    • Dynorphin A(1-13) antibody
    • Dynorphin A(1-17) antibody
    • Dynorphin A(1-8) antibody
    • Dynorphin B antibody
    • Dynorphin B(1-13) antibody
    • Dynorphin B-29 antibody
    • Enkephalin B antibody
    • Leu enkephalin antibody
    • Leumorphin antibody
    • Neoendorphin dynorphin enkephalin prepropeptide antibody
    • PDYN antibody
    • PDYN_HUMAN antibody
    • PENKB antibody
    • Preprodynorphin antibody
    • Preproenkephalin B antibody
    • Prodynorphin antibody
    • Proenkephalin B antibody
    • Rimorphin antibody
    • SCA23 antibody
    see all

Anti-ProDynorphin antibody 画像

  • ab11137 staining ProDynorphin in Rat brain tissue by Immunohistochemistry - Free floating sections. The tissue was fixed by transcardial perfusion with 4% paraformaldehyde, 0.1% glutaraldeyde in 0.1M phosphate buffer (pH 7.4). The brain was postfixed with 4% paraformaldehyde in 0.1 M phosphate buffer (pH7.4) overnight at 4°C and then cut into 50 µm-thick parasagittal sections with a vibrating microtome. The primary antibody was diluted 1/100 in and incubated with the sample for 16 hours at 20°C. A Cy3®-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500.

    See Abreview

プロトコール

Anti-ProDynorphin antibody (ab11137) 使用論文

This product has been referenced in:
  • Romero-Picó A  et al. Hypothalamic ?-opioid receptor modulates the orexigenic effect of ghrelin. Neuropsychopharmacology 38:1296-307 (2013). Read more (PubMed: 23348063) »
  • Schlosburg JE  et al. Long-term antagonism of ? opioid receptors prevents escalation of and increased motivation for heroin intake. J Neurosci 33:19384-92 (2013). Rat . Read more (PubMed: 24305833) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formalin
Username

Allisa Song

Verified customer

投稿 Feb 26 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA+NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Cortex)
Specification
Cortex
Permeabilization
Yes - 0.3% Triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 18 2014

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain
Username

Dr. Kouichi Nakamura

Verified customer

投稿 Mar 24 2011

I have contacted our accounting department and you will be issued a refund for your order for ab11137. This process does take a couple of weeks and if you have any questions regarding the refund please email: accounts@abcam.com If you have any future ...

Read More

Thank you for your patience and the details that you have provided us with. To our knowledge, this antibody has only been tested for application in IHC-frozen sections. We do not know how the antibody works in paraformaldehyde or formalin-fixed section...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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