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Recombinant fragment - N terminal pro-peptide (amino acids 26-181) of human collagen type II.
Our Abpromise guarantee covers the use of ab17771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
ab17771 staining mouse eye tissue sections -- conjunctiva and limbus by IHC-P. The tissue was fixed with paraformaldehyde and antigen retrieval was heat mediated using citrate buffer. The blocking agent was 1% TSA at 25°C for 1 hour. The primary antibody was incubated (undiluted) at 25°C for 15 hours. A biotinylated anti-mouse was used as the secondary antibody.