Application
Western blot
Sample
Human Cell lysate - other (human fibroblasts, chondrocytes,iPSC)
Loading amount
30 µg
Specification
human fibroblasts, chondrocytes,iPSC
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C
Secondary antibody
Name
Non-Abcam antibody was used: SIGMA Anti-mouse IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
West fento
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 150, 220 kDa
Additional data
Additional Notes
DISCOUNT CODE: ABTRIAL-TBDPN
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 21 2013