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Our Abpromise guarantee covers the use of ab13115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 13 kDa.|
|IP||Use at an assay dependent concentration. PubMed: 21482747|
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [24E11] to proBNP (HRP) (ab13123). Can be used as capture antibody in conjunction with recommended pair. Recognises the antigen in blood of patients with heart faliure, unstable angina and myocardial infarction.|
proBNP was immunoprecipitated from 7x106 HCT116 (human colon carcinoma cell line) cells with ab26 at 1/150 dilution. Western blot was performed from the immunoprecipitate using anti-p53 antibody. Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175739) was used as secondary antibody at 1/5000 dilution.
Lane 1: HCT116 whole cell lysate 10 µg (Input).
Lane 2: ab207799 IP inetoposide treated HCT116 whole cell lysate.
Lane 3: ab207799 IP inetoposide treated HCT116 p53-/- whole cell lysate (negative control).