製品の概要

  • 製品名Anti-pro Caspase 9 antibody [E84]
    pro Caspase 9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E84] to pro Caspase 9
  • 特異性This antibody only recognises the pro form of Caspase 9 (46kDa)
  • アプリケーション適用あり: WB, Flow Cyt, IHC-Pmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near N-terminus of human Caspase-9

  • ポジティブ・コントロール
    • Jurkat cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名E84
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/25.
  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
      Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
    • 組織特異性Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
    • 配列類似性Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • 発生段階Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
    • 翻訳後修飾Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
      Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • APAF-3 antibody
      • APAF3 antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase-9 subunit p10 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • ICELAP6 antibody
      • MCH6 antibody
      see all

    Anti-pro Caspase 9 antibody [E84] 画像

    • Anti-pro Caspase 9 antibody [E84] (ab32068) at 1/2000 dilution + Jurkat cell lysate at 10 µg

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068).

    Anti-pro Caspase 9 antibody [E84] (ab32068) 使用論文

    This product has been referenced in:
    • Catuogno S  et al. miR-34c may protect lung cancer cells from paclitaxel-induced apoptosis. Oncogene : (2012). WB ; Human . Read more (PubMed: 22370637) »
    • Hou W  et al. Autophagic degradation of active caspase-8: a crosstalk mechanism between autophagy and apoptosis. Autophagy 6:891-900 (2010). WB . Read more (PubMed: 20724831) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab32068.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"