Anti-pro Caspase-7 抗体 [Y33] (ab32067)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y33] to pro Caspase-7
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-pro Caspase-7 antibody [Y33]
pro Caspase-7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y33] to pro Caspase-7 -
由来種
Rabbit -
特異性
The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7. -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Jurkat cell lysate and human lung squamous carcinoma.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y33 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32067の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
|
|
IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
組織特異性
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
-
別名
- apoptosis-related cysteine peptidase antibody
- Apoptotic protease Mch-3 antibody
- CASP-7 antibody
see all
画像
-
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)
Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and the pro Caspase 7 knockout samples were subjected to SDS-PAGE. Ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Anti-pro Caspase-7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate
Predicted band size: 34 kDa
Observed band size: 34 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab32067 は 2 報の論文で使用されています。
- Zhang W et al. Paired box 5 increases the chemosensitivity of esophageal squamous cell cancer cells by promoting p53 signaling activity. Chin Med J (Engl) 135:606-618 (2022). PubMed: 35191417
- Yuan Y et al. Suppression of tumor cell proliferation and migration by human umbilical cord mesenchymal stem cells: A possible role for apoptosis and Wnt signaling. Oncol Lett 15:8536-8544 (2018). PubMed: 29805590