製品の概要

  • 製品名
    Anti-pro Caspase-12 antibody
    pro Caspase-12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to pro Caspase-12
  • 特異性
    The antibody only recognizes the prodomain, not the activated form.
  • アプリケーション
    適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    非交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Mouse pro Caspase-12 aa 2-17 (N terminal).
    Sequence:

    AARRTHERDPIYKIKG


    Database link: Q6UXS9
    (Peptide available as ab8375)

  • ポジティブ・コントロール
    • Mouse spleen tissue lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa.Can be blocked with Mouse Caspase-12 peptide (ab8375).

ターゲット情報

  • 関連性
    Caspase 12 is activated by ER stress, including disruption of ER calcium homeostasis and mediates ER stress-induced apoptosis. Caspase 12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase 12 mediates cytotoxicity induced by amyloid-beta. It is ubiquitously expressed in mouse tissue.
  • 細胞内局在
    Endoplasmic reticulum
  • 参照データベース
  • 別名
    • CASP12 antibody
    • CASP12P1 antibody

画像

  • All lanes : Anti-pro Caspase-12 antibody (ab8118) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Mouse spleen tissue lysate
  • ab8118 at 10µg/ml staining Caspase-12 in human heart tissue by IHC

参考文献

This product has been referenced in:
  • Hauri-Hohl MM  et al. Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation. Blood 109:4080-8 (2007). Read more (PubMed: 17213290) »

See 1 Publication for this product

レビューと Q&A

Thank you for your e-mail. Did you mean ab24852, rather than 24582? The antibodies are against caspase 12, not caspase 1. Can you please confirm that the customer is looking for caspase 12 and not caspase 1? I compared the two sheets of ab24852 and...

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I'm sorry to hear your customer is experiencing problems with ab8118. The customer mentions that he/she provided images however these did not reach us, so I cannot see the membrane pattern with this antibody I'm afraid. It is very unclear what ...

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This antibody is raised against the N-terminal sequence AARRTHERDPIYKIKG. If this sequence forms part of the pro-peptide, then this antibody will be selective for the non-activated form. The blocking peptide for this antibody is available separatel...

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These two antibodies ab8118 and ab8117 recognize the pre-activated form of caspase 12, however we have not tested for the activated/cleaved form.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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