The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Caspases are a family of cytosolic aspartate specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase 10 (MCH4, FLICE2) is one of the initiator caspases, and is localized to various tissues. Pro Caspase 10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis. This death domain complex cleaves pro Caspase 10 into a large active fragment and a small fragment. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis.