Anti-pro Caspase-10 抗体 [E35] (ab32155)
Key features and details
- Rabbit monoclonal [E35] to pro Caspase-10
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-pro Caspase-10 antibody [E35]
pro Caspase-10 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E35] to pro Caspase-10 -
由来種
Rabbit -
特異性
The antibody does not crossreact with other Caspase family members. -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human pro Caspase-10 aa 200-300 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Hela cell lysate. ICC/IF and FACS: Hela cells. IHC-P: Human breast carcinoma tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
E35 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32155の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100.
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WB |
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/100. |
WB
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
Caspases are a family of cytosolic aspartate specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase 10 (MCH4, FLICE2) is one of the initiator caspases, and is localized to various tissues. Pro Caspase 10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis. This death domain complex cleaves pro Caspase 10 into a large active fragment and a small fragment. Cleaved Caspase 10 leads to the processing of Caspase 3 and Caspase 7, initiating a caspase cascade and subsequent apoptosis. -
参照データベース
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q8IUP5 Human
- SwissProt: Q92851 Human
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別名
- Apoptotic protease Mch4 antibody
- CASP10 antibody
- Caspase 10 antibody
see all
画像
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Anti-pro Caspase-10 antibody [E35] (ab32155) at 1/1000 dilution + Hela cell lysate
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Ab32155, at 1/50 dilution, staining pro Caspase 10 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Ab32155, at 1/100 dilution, staining pro Caspase 10 in apoptotic HeLa cells by Immunofluorescence.
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Intracellular flow cytometric analysis of permeabilized HeLa cells using ab32155 at a 1/50 dilution (red) or a rabbit IgG (negative) (green).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab32155 は 4 報の論文で使用されています。
- Wang Y et al. The relationship between liver pathological inflammation degree and pyroptosis in chronic hepatitis B patients. J Med Virol N/A:N/A (2021). PubMed: 34061368
- Han X et al. Zika virus infection induced apoptosis by modulating the recruitment and activation of pro-apoptotic protein Bax. J Virol 95:N/A (2021). PubMed: 33536166
- Lu Z et al. Dracorhodin perchlorate induces apoptosis and G2/M cell cycle arrest in human esophageal squamous cell carcinoma through inhibition of the JAK2/STAT3 and AKT/FOXO3a pathways. Mol Med Rep 20:2091-2100 (2019). PubMed: 31322237
- Coutinho-Camillo CM et al. Caspase expression in oral squamous cell carcinoma. Head Neck 33:1191-8 (2011). IHC ; Human . PubMed: 21755562