製品の概要

  • 製品名Anti-PRMT6 antibody
    PRMT6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PRMT6
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to Human PRMT6 (C terminal). A region between residue 325 and the C-terminus (residue 375) of PRMT6 (AAK85733.1).
    Database link: 55170

  • ポジティブ・コントロール
    • Whole cell lysate from 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC).
    • 組織特異性Highly expressed in kidney and testis.
    • 配列類似性Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT6 subfamily.
      Contains 1 SAM-dependent MTase PRMT-type domain.
    • 翻訳後修飾Automethylation enhances its stability and antiretroviral activity.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ANM6_HUMAN antibody
      • Chromobox protein homolog 7 antibody
      • FLJ10559 antibody
      • FLJ51477 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6 antibody
      • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6 antibody
      • Histone-arginine N-methyltransferase PRMT6 antibody
      • HMT1 hnRNP methyltransferase like 6. antibody
      • HRMT1L6 antibody
      • OTTHUMP00000012633 antibody
      • PRMT 6 antibody
      • prmt6 antibody
      • Protein arginine methyltransferase 6 antibody
      • Protein arginine N methyltransferase 6 antibody
      • Protein arginine N-methyltransferase 6 antibody
      see all

    Anti-PRMT6 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRMT6 with ab72205 at 1/5000 (0.2 µg/ml). Detection: DAB.
    • All lanes : Anti-PRMT6 antibody (ab72205) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 42 kDa

    Anti-PRMT6 antibody (ab72205) 使用論文

    ab72205 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (u2os)
    Loading amount 80 µg
    Specification u2os
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Jun 10 2011

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cultured Cells (u2os)
    Specification u2os
    Fixative Formaldehyde
    Permeabilization Yes - triton
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Dec 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"