製品の概要

  • 製品名Anti-PRMT5 antibody [PRMT5-21]
    PRMT5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PRMT5-21] to PRMT5
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Monkey
  • 免疫原

    Antibody was produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with PRMT5 containing the C-terminal region of the Human PRMT5 (amino acids 315-637).

  • エピトープMonoclonal Anti-PRMT5 (protein-arginine methyl transferase 5) recognizes an epitope within the C-terminal region of PRMT5.
  • ポジティブ・コントロール
    • Whole cell extract of human melanoma cell line G361.
  • 特記事項If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 2 µg/ml (determined by using a whole cell extract of human melanoma cell line G361). Predicted molecular weight: 70 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the protein arginine N-methyltransferase family.
    • 翻訳後修飾Disulfide bonds and non-covalent association mediate homooligomers formation.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 72 kDa ICln binding protein antibody
      • 72 kDa ICln-binding protein antibody
      • ANM5_HUMAN antibody
      • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
      • Histone-arginine N-methyltransferase PRMT5 antibody
      • HMT1 hnRNP methyltransferase like 5 antibody
      • HOMOLOG OF; SKB1 antibody
      • HRMT1L5 antibody
      • IBP72 antibody
      • Jak-binding protein 1 antibody
      • JBP 1 antibody
      • JBP1 antibody
      • PRMT 5 antibody
      • PRMT5 antibody
      • Protein arginine methyltransferase 5 antibody
      • Protein arginine N methyltransferase 5 antibody
      • Protein arginine N methyltransferase 5 N terminally processed antibody
      • Protein arginine N-methyltransferase 5 antibody
      • S. POMBE antibody
      • S. POMBE HOMOLOG OF; SKB1 antibody
      • SHK1 KINASE BINDING PROTEIN 1 antibody
      • Shk1 kinase binding protein 1 homolog antibody
      • Shk1 kinase-binding protein 1 homolog antibody
      • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
      • SKB 1 antibody
      • SKB1 antibody
      • SKB1 homolog antibody
      • SKB1: SKB1 homolog (S. pombe) antibody
      • SKB1Hs antibody
      see all

    Anti-PRMT5 antibody [PRMT5-21] (ab12191) 使用論文

    This product has been referenced in:
    • van Bergeijk J  et al. The spinal muscular atrophy gene product regulates neurite outgrowth: importance of the C terminus. FASEB J 21:1492-502 (2007). Read more (PubMed: 17317728) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"