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Our Abpromise guarantee covers the use of ab31751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19584108|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).Can be blocked with Human PRMT5 peptide (ab30553).
ab31751 shows weak staining in mouse samples.
|IHC-P||1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Image courtesy of Human Protein Atlas
ab31751 staining PRMT5 in human prostate, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab31751 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31751 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for PRMT5 (red) using ab31751 (0.5 µg/ml) in ICC/IF.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"