製品の概要

  • 製品名
    Anti-PRMT5 antibody - C-terminal
    PRMT5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PRMT5 - C-terminal
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide within Human PRMT5 (C terminal). The exact sequence is proprietary.
    Database link: O14744
    (Peptide available as ab30553)

  • ポジティブ・コントロール
    • WB: HL60, HEK-293 and NIH/3T3 whole cell lysate. IHC-P: Human prostate tissue. ICC/IF: U-2 OS cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 19584108
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).Can be blocked with Human PRMT5 peptide (ab30553).

ab31751 shows weak staining in mouse samples.

IHC-P 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the protein arginine N-methyltransferase family.
  • 翻訳後修飾
    Disulfide bonds and non-covalent association mediate homooligomers formation.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 72 kDa ICln binding protein antibody
    • 72 kDa ICln-binding protein antibody
    • ANM5_HUMAN antibody
    • Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
    • Histone-arginine N-methyltransferase PRMT5 antibody
    • HMT1 hnRNP methyltransferase like 5 antibody
    • HOMOLOG OF; SKB1 antibody
    • HRMT1L5 antibody
    • IBP72 antibody
    • Jak-binding protein 1 antibody
    • JBP 1 antibody
    • JBP1 antibody
    • PRMT 5 antibody
    • PRMT5 antibody
    • Protein arginine methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 antibody
    • Protein arginine N methyltransferase 5 N terminally processed antibody
    • Protein arginine N-methyltransferase 5 antibody
    • S. POMBE antibody
    • S. POMBE HOMOLOG OF; SKB1 antibody
    • SHK1 KINASE BINDING PROTEIN 1 antibody
    • Shk1 kinase binding protein 1 homolog antibody
    • Shk1 kinase-binding protein 1 homolog antibody
    • Shk1 kinase/binding protein 1, S. pombe, homolog of antibody
    • SKB 1 antibody
    • SKB1 antibody
    • SKB1 homolog antibody
    • SKB1: SKB1 homolog (S. pombe) antibody
    • SKB1Hs antibody
    see all

画像

  • All lanes : Anti-PRMT5 antibody - C-terminal (ab31751) at 1 µg/ml

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney cell line) whole cell lysate
    Lane 3 : NIH 3T3 whole cell lysate (ab7179)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes
  • Image courtesy of Human Protein Atlas

    ab31751 staining PRMT5 in human prostate, showing a distinct and strong staining pattern in glandular cells. Paraffin embedded human skin tissue was incubated with ab31751 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab31751 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for PRMT5 (red) using ab31751 (0.5 µg/ml) in ICC/IF.

    See Abreview

参考文献

This product has been referenced in:
  • Webb LM  et al. PRMT5-Selective Inhibitors Suppress Inflammatory T Cell Responses and Experimental Autoimmune Encephalomyelitis. J Immunol 198:1439-1451 (2017). WB ; Mouse . Read more (PubMed: 28087667) »
  • Mizuno K & Sloboda RD Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption. Mol Biol Cell 28:1208-1222 (2017). Read more (PubMed: 28298486) »

See all 8 Publications for this product

レビューと Q&A

This antibody has been tested on whole cell lysates only.
I hope this is helpful. Please contact us again if you have any further questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (u2os)
Loading amount
80 µg
Specification
u2os
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (u2os)
Specification
u2os
Fixative
Formaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
60 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (12,5)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Mr. Jörg Hoehfeld

Verified customer

投稿 Mar 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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