製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PRMT2
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: AIADYAATDE TQLSFLRGEK ILILRQTTAD WWWGERAGCC GYIPANHVGK, corresponding to a region within N terminal amino acids 37-86 of Human PRMT2

  • ポジティブ・コントロール
    • Human liver tissue and jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.625 µg/ml. Detects a band of approximately 35,36, 40, 48, 62 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
  • 組織特異性
    Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
  • 配列類似性
    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 SH3 domain.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocates from the cytoplasm to the nucleus, after hormone exposure.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANM2_HUMAN antibody
    • EC 2.1.1. antibody
    • histone arginine N methyltransferase PRMT2 antibody
    • Histone-arginine N-methyltransferase PRMT2 antibody
    • HMT 1 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 1 antibody
    • HMT1 antibody
    • HMT1 hnRNP methyltransferase like 1 antibody
    • hnRNP methyltransferase (S. cerevisiae) like 1 antibody
    • hnRNP methyltransferase like 1 antibody
    • Hrmt1l1 antibody
    • MGC111373 antibody
    • PRMT 2 antibody
    • PRMT2 alpha antibody
    • prmt2 antibody
    • PRMT2 beta antibody
    • PRMT2 gamma antibody
    • PRMT2 protein antibody
    • PRMT2L2 antibody
    • Protein arginine methyltransferase 2 antibody
    • Protein arginine N methyltransferase 2 antibody
    • Protein arginine N-methyltransferase 2 antibody
    • Zf2 antibody
    see all

画像

  • Rabbit Anti-PRMT2 Antibody ab66763. Paraffin Embedded Tissue: Human Liver. Cellular Data: Liver cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
  • Anti-PRMT2 antibody (ab66763) at 0.625 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 35 kDa,36 kDa,40 kDa,62 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab66763 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PRMT2 antibody (ab66763) at 1/1000 dilution

    Lane 1 : Lysate prepared from human LCL.
    Lane 2 : Lysate prepared from murine brain.


    Predicted band size : 48 kDa

参考文献

ab66763 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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