製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PRMT2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human PRMT2 aa 344-375 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • WB: HL60 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 一次抗体 備考
    Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone-2A, 3, 4), IL enhancer binding factor, nuclear factors (NF-45, -90, ILF3, Nucleolin, STAT1, Poly(A)-binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src-adaptor Sam68), apoptosis proteins (FADD, ICE-like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 48 kDa.

ターゲット情報

  • 機能
    Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
  • 組織特異性
    Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
  • 配列類似性
    Belongs to the protein arginine N-methyltransferase family.
    Contains 1 SH3 domain.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocates from the cytoplasm to the nucleus, after hormone exposure.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANM2_HUMAN antibody
    • EC 2.1.1. antibody
    • histone arginine N methyltransferase PRMT2 antibody
    • Histone-arginine N-methyltransferase PRMT2 antibody
    • HMT 1 antibody
    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 1 antibody
    • HMT1 antibody
    • HMT1 hnRNP methyltransferase like 1 antibody
    • hnRNP methyltransferase (S. cerevisiae) like 1 antibody
    • hnRNP methyltransferase like 1 antibody
    • Hrmt1l1 antibody
    • MGC111373 antibody
    • PRMT 2 antibody
    • PRMT2 alpha antibody
    • prmt2 antibody
    • PRMT2 beta antibody
    • PRMT2 gamma antibody
    • PRMT2 protein antibody
    • PRMT2L2 antibody
    • Protein arginine methyltransferase 2 antibody
    • Protein arginine N methyltransferase 2 antibody
    • Protein arginine N-methyltransferase 2 antibody
    • Zf2 antibody
    see all

画像

  • Anti-PRMT2 antibody (ab3667) + HL60 whole cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size : 48 kDa
  • All lanes : Anti-PRMT2 antibody (ab3667)

    Lane 1 : 293 cell lysate - nontransfected
    Lane 2 : 293 cell lysate - transfected with PRMT2

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 48 kDa

プロトコール

参考文献

This product has been referenced in:
  • Herrmann F  et al. Human protein arginine methyltransferases in vivo--distinct properties of eight canonical members of the PRMT family. J Cell Sci 122:667-77 (2009). WB ; Human . Read more (PubMed: 19208762) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (u2os)
Loading amount
80 µg
Specification
u2os
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Jun 10 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (u2os)
Specification
u2os
Fixative
Formaldehyde
Permeabilization
Yes - triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

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Verified customer

投稿 Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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