The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
Belongs to the protein arginine N-methyltransferase family.
HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody
HMT1 hnRNP methyltransferase antibody
HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody
HMT1 hnRNP methyltransferase like 2 antibody
HRMT1 L2 antibody
HRMT1L 2 antibody
Human mRNA for suppressor for yeast mutant antibody
Human mRNA for suppressor for yeast mutant complete cds antibody
Interferon receptor 1 bound protein 4 antibody
Interferon receptor 1 bound protein4 antibody
Interferon receptor 1-bound protein 4 antibody
Interferon receptor 1bound protein 4 antibody
IR1 B4 antibody
IR1B 4 antibody
Mrmt 1 antibody
PRMT 1 antibody
Protein arginine methyltransferase 1 antibody
Protein arginine N methyltransferase 1 antibody
Protein arginine N methyltransferase1 antibody
Protein arginine N-methyltransferase 1 antibody
S. cerevisiae like 2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody (ab84361)This image is courtesy of an anonymous Abreview
ab84361 staining PRMT1 in mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in blocking solution (PBS-T + 1% BSA)) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Detection of Human PRMT1 in formalin-fixed paraffin-embedded section of Human prostate adenocarcinoma by Immunohistochemistry using ab84361 at a dilution of 1/250. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.
Detection of Mouse PRMT1 in formalin-fixed paraffin-embedded section of Mouse squamous cell carcinoma by Immunohistochemistry using ab84361 at a dilution of 1/250. Detection was performed by DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.