製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PRKCDBP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to internal amino acids 178-190 (

    C-RRTGLQKVQSLR

    ) of Human PRKCDBP (NP_659477.2).

  • ポジティブ・コントロール
    • Human Adipose and NIH3T3 cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 28 kDa.

ターゲット情報

  • 機能
    Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2. Seems to have an immune potentiation function.
  • 組織特異性
    Strongly expressed in mammary and epithelial cells.
  • 配列類似性
    Belongs to the PTRF/SDPR family.
  • 翻訳後修飾
    In vitro, phosphorylated by PRKCD.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cavin 3 antibody
    • Cavin-3 antibody
    • CAVIN3 antibody
    • hSRBC antibody
    • MGC20400 antibody
    • PRDBP_HUMAN antibody
    • Prkcdbp antibody
    • Protein kinase C delta binding protein antibody
    • Protein kinase C delta-binding protein antibody
    • Sdr related gene product that binds to c kinase antibody
    • Serum deprivation response factor related gene product that binds to C kinase antibody
    • Serum deprivation response factor-related gene product that binds to C-kinase antibody
    • SRBC antibody
    see all

画像

  • All lanes : Anti-PRKCDBP antibody (ab99427) at 0.3 µg/ml

    Lane 1 : Mouse ovary lysates
    Lane 2 : Rat uterus lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 28 kDa
  • Anti-PRKCDBP antibody (ab99427) at 0.1 µg/ml + Adipose cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 28 kDa
    Primary incubation was 1 hour.

    Detected by chemiluminescence.

プロトコール

参考文献

This product has been referenced in:
  • Mohan J  et al. Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae. J Cell Sci 128:979-91 (2015). Read more (PubMed: 25588833) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (adipose)
Loading amount
10 µg
Specification
adipose
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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投稿 May 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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