製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PRKCDBP
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the center region of human PRKCDBP

  • ポジティブ・コントロール
    • A2058 cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    ELISA: 1/1000.
    WB: 1/50 - 1/100. Predicted molecular weight: 28 kDa.
    Bands at 40-45 kDa may also occur due to 2 possible prenylation sites

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2. Seems to have an immune potentiation function.
    • 組織特異性
      Strongly expressed in mammary and epithelial cells.
    • 配列類似性
      Belongs to the PTRF/SDPR family.
    • 翻訳後修飾
      In vitro, phosphorylated by PRKCD.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cavin 3 antibody
      • Cavin-3 antibody
      • CAVIN3 antibody
      • hSRBC antibody
      • MGC20400 antibody
      • PRDBP_HUMAN antibody
      • Prkcdbp antibody
      • Protein kinase C delta binding protein antibody
      • Protein kinase C delta-binding protein antibody
      • Sdr related gene product that binds to c kinase antibody
      • Serum deprivation response factor related gene product that binds to C kinase antibody
      • Serum deprivation response factor-related gene product that binds to C-kinase antibody
      • SRBC antibody
      see all

    画像

    • Anti-PRKCDBP antibody (ab76676) at 1/50 dilution + A2058 cell lysate at 35 µg

      Predicted band size : 28 kDa
      Observed band size : 29 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    This product has been referenced in:
    • Sathish V  et al. Inflammation, caveolae and CD38-mediated calcium regulation in human airway smooth muscle. Biochim Biophys Acta 1843:346-51 (2014). Read more (PubMed: 24275509) »

    See 1 Publication for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Lung)
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

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    Verified customer

    投稿 Nov 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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