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RabMAb

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) 抗体 [EPR5671] (ab124709)

製品の概要

  • 製品名
    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671]
  • 製品の詳細
    Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • 特異性
    This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.
  • アプリケーション
    適用あり: WB, Flow Cytmore details
    適用なし: ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues surrounding Threonine 718 of Human PKN3 (Q6P5Z2), Threonine 816 of Human PKN2 (Q16513), and Threonine 774 of Human PKN1 (Q16512) proteins.

  • ポジティブ・コントロール
    • HeLa cell lysates and Hek 293 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 104, 112, 99 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for ICC/IF,IHC-P or IP.
  • 画像

    • All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 104, 112, 99 kDa
    • ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
      Green: untreated cells
      Red: Okadaic Acid + Calyculin A-treated cells
      Blue: untreated cells preincubated with the immunizing phosphopeptides
      Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .

    参考文献

    ab124709 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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