Prism Ultra Protein Ladder (10-245 kDa) (ab116028)

製品の概要

  • 製品名Prism Ultra Protein Ladder (10-245 kDa)
    Prestained Protein Ladder ihc 試薬 製品一覧
  • アプリケーション適用あり: SDS-PAGE, WBmore details
  • 特記事項

    ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

    ab116028 is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

    Key product features:
    • Broad range: 10-245 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 - 2.5µl

    Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

製品の特性

  • 別名
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder

アプリケーション

Our Abpromise guarantee covers the use of ab116028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
SDS-PAGE Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
WB Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

Prism Ultra Protein Ladder (10-245 kDa) 画像

  • Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer.Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

Prism Ultra Protein Ladder (10-245 kDa) (ab116028) 使用論文

This product has been referenced in:
  • Alshammari EM  et al. Optimization of Extraction Parameters for Enhanced Production of Ovotransferrin from Egg White for Antimicrobial Applications. Biomed Res Int 2015:934512 (2015). Read more (PubMed: 26640801) »

See 1 Publication for this product

Product Wall

One of the best Pre stained protein ladder

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I have used 1.8 ul per well and get a clear band on transfer to membrane.
Username

Vicky

Verified customer

投稿 Oct 10 2016

The apparent sizes will be the same as the ones in the second gel on the online datasheet, which is a 10% Bis-Tris gel, MOPS running buffer. The distribution will be different, with a little more separation of the higher molecular weight proteins at th...

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I can confirm that ab116027, ab116028, and ab116029 do not contain histone tagged proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"