Prism Ultra Protein Ladder (10 - 180 kDa) (ab116027)


  • 製品名
    Prism Ultra Protein Ladder (10 - 180 kDa)
    Prestained Protein Ladder ihc 試薬 製品一覧
  • アプリケーション
    適用あり: WB, SDS-PAGEmore details
  • 特記事項

    ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

    ab116027 is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.

    Do not heat, dilute, add reducing agent before loading.

    Key product features:

    • Broad range: 10-180 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 - 2.5µl

    Storage: Stable at +25°C for 2 weeks Stable at +4°C for 3 months Store at -20°C for 24 months


  • 製品の状態
  • 保存方法
    Please see notes section.
  • バッファー
    Constituents: 0.44% Tris citrate/phosphate, 2% SDS, 0.02% DTT, 0.02% Urea, 15% Glycerol
  • Concentration information loading...
  • 研究分野
  • 別名
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder


Our Abpromise guarantee covers the use of ab116027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transfering. Apply more for thicker (>1.5 mm) or larger gel.
SDS-PAGE Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.


  • Gel 1: Tris-Glycine (~4-20%) Gel 2: Bis-Tris (12%) MOPS buffer Gel 3: Bis-Tris (10%) MES bufferPre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.


This product has been referenced in:
  • Fiserova J  et al. Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. J Cell Sci 127:124-36 (2014). Read more (PubMed: 24144701) »

See 1 Publication for this product

レビューと Q&A

Excellent protein marker for immunoblots

Excellent Excellent 5/5 (Ease of Use)
Very easy to use protein ladder in the range from 10-180 kDa. Good separation in SDS-PAGE and nice transfer onto nitrocellulose membrane. The gel used was a 10% Tris-Bis gel from life-technologies. the protein ladder was used at 2.5ul per well.

Abcam user community

Verified customer

投稿 Jan 29 2015

Merci pour votre appel.

Je peux vous confirmer que nos marqueurs de poids moléculaires, ab116027, ab116028 ou ab116029 ne contiennent aucune protéine avecun His tag.

Je tiens aussi à vous signaler, que des fois,...

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