Anti-Presenilin 1 (phospho S310) 抗体 [EP2001Y] (ab76131)

製品の概要

  • 製品名Anti-Presenilin 1 (phospho S310) antibody [EP2001Y]
    Presenilin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2001Y] to Presenilin 1 (phospho S310)
  • 特異性ab76131 detects Presenilin 1 phosphorylated on serine 310.
  • アプリケーション適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 310 of human Presenilin 1.

  • ポジティブ・コントロール
    • Jurkat cell lysates untreated or treated with TPA.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC
  • 追加情報ICC: 1/100 - 1/250.
    WB: 1/5000 - 1/10000. Detects a band of approximately 23 kDa (CTF Fragment) (predicted molecular weight: 53 kDa).

    Is unsuitable for Flow Cyt, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
    • 組織特異性Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
    • 関連疾患Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
      Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
      Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
    • 配列類似性Belongs to the peptidase A22A family.
    • ドメインThe PAL motif is required for normal active site conformation.
    • 翻訳後修飾Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
      After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
    • 細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AD3 antibody
      • Ad3h antibody
      • FAD antibody
      • Homo Sapiens Clone CC44 Senilin 1 antibody
      • Presenilin-1 CTF12 antibody
      • Protein S182 antibody
      • PS 1 antibody
      • PS-1 antibody
      • PS1-CTF12 antibody
      • PSEN1 antibody
      • PSN1_HUMAN antibody
      • PSNL1 antibody
      • S182 antibody
      see all

    Anti-Presenilin 1 (phospho S310) antibody [EP2001Y] 画像

    • All lanes : Anti-Presenilin 1 (phospho S310) antibody [EP2001Y] (ab76131) at 1/5000 dilution

      Lane 1 : Jurkat cell lysates untreated
      Lane 2 : Jurkat cell lysates treated with 200 nM TPA, 30 minutes at 37C, serum-free.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)

    Anti-Presenilin 1 (phospho S310) antibody [EP2001Y] (ab76131) 使用論文

    ab76131 has not yet been referenced specifically in any publications.

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