製品の概要

  • 製品名Anti-PREI3 antibody
    PREI3 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PREI3
  • 特異性ab114057 is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human PREI3 aa 137-151 (internal sequence).
    Sequence:

    C-NSNKYFPSRVSIKES


    Database link: Q9Y3A3

  • ポジティブ・コントロール
    • Human Brain (Frontal Cortex), Mouse and Rat Brain lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab114057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:4,000.

WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-PREI3 antibody 画像

  • All lanes : Anti-PREI3 antibody (ab114057) at 0.3 µg/ml

    Lane 1 : Mouse Brain lysate
    Lane 2 : Rat Brain lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 26 kDa
  • ab114057 (5µg/ml) shows staining of PREI3 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows cytoplasm staining in the neuronal bodies.

  • Anti-PREI3 antibody (ab114057) at 0.3 µg/ml + Human Brain, Frontal Cortex lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Primary incubation was 1 hour.

Anti-PREI3 antibody (ab114057) 使用論文

ab114057 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"