製品の概要

  • 製品名Anti-PREB antibody
    PREB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PREB
  • 特異性This antibody is specific for PREB.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: RVENLQAVQT DFSSDPLQKV VCFNHDNTLL ATGGTDGYVR VWKVPSLEKV, corresponding to amino acids 140-189 of Human PREB

  • ポジティブ・コントロール
    • Raji cell lysate and human heart tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab42501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 41, 30 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.
  • 組織特異性Ubiquitous.
  • 配列類似性Contains 3 WD repeats.
  • 細胞内局在Nucleus. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mammalian guanine nucleotide exchange factor mSec12 antibody
    • Preb antibody
    • PREB_HUMAN antibody
    • Prolactin regulatory binding element protein antibody
    • Prolactin regulatory element binding antibody
    • Prolactin regulatory element binding protein antibody
    • Prolactin regulatory element-binding protein antibody
    • SEC12 antibody
    see all

Anti-PREB antibody 画像

  • Anti-PREB antibody (ab42501) at 0.5 µg/ml + Raji cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 41& 30 kDa (why is the actual band size different from the predicted?)
  • ab42501 at 4µg/ml staining PREB in human myocardial cells.

Anti-PREB antibody (ab42501) 使用論文

ab42501 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab42501.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"