製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PREB
  • 特異性
    Detects recombinant mouse PREB (Prolactin Regulatory Element Binding protein).
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant fragment corresponding to Mouse PREB.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

  • 機能
    Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Contains 3 WD repeats.
  • 細胞内局在
    Nucleus. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mammalian guanine nucleotide exchange factor mSec12 antibody
    • Preb antibody
    • PREB_HUMAN antibody
    • Prolactin regulatory binding element protein antibody
    • Prolactin regulatory element binding antibody
    • Prolactin regulatory element binding protein antibody
    • Prolactin regulatory element-binding protein antibody
    • SEC12 antibody
    see all

画像



  • Predicted band size : 45 kDa

    Western blot detection of recombinant mouse PREB using ab3422.

  • ab3422 staining PREB in mouse skeletal tissue sections by Immunohistochemistry (Frozen sections)

    Tissue was fixed with acetone, blocked with 10% serum for 1 hour at 22°C and permeabilized with 1% Triton X-100. Samples were incubated with primary antibody (1/50 in 10% serum) for 12 hours at 4°C. A Rhodamine red-x conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

プロトコール

参考文献

ab3422 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skeletal Muscle)
Specification
Skeletal Muscle
Fixative
Acetone
Permeabilization
Yes - 1% Triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

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投稿 Jun 28 2012

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